By skyforbes 
Principle
Antistreptolysin ‘O’ (ASO) test kit is based on the
following principle Polystyrene latex particles are coated with purified and
stabilized Streptolysin O (antigen), which reacts with its corresponding
Antistreptolysin O (antibody). in the test sample resulting in the
agglutination of latex particles. Results are expressed in IU/mL of
anti-Streptolysin O (ASO) based on the WHO International Standard.
Specimen: Serum or plasma.
Requirement
- ASO Latex Reagent.
- Positive Control Serum.
- Negative Control Serum.
- Glass Slide.
- Disposable Applicator Sticks.
- Disposable Plastic Droppers.
- Rubber Teats.
Procedure
A. QUALITATIVE SLIDE TEST
Bring test sera and reagents to room temperature (+15 °C to
+30 °C) before testing and mix gently prior to use.
with the help of disposable plastic dropper place one drop
of undiluted Test serum within the circled area on the special slide provided
in the kit.
Add one drop of well mixed ASO Latex Reagent.
Mix the reagent and serum using the applicator stick and
spread within The entire circle of the glass slide. 5) Slowly rock the slide and observe for
macroscopic agglutination. Do not observe for longer than 2 minutes.
B. SEMI QUANTITATIVE SLIDE TEST
1) All samples giving positive results in the qualitative
test should be tested by Semi-quantitative test or Hemolysin titration test.
2)Prepare dilutions of the serum sample with normal saline
as suggested in The following table Serum Dilution Corresponding IU/mL
3)With the help of disposable plastic dropper, dispense one
drop of diluted serum into one of the circled area on the special slide
provided.
4) Add one drop of well mixed ASO Latex Reagent.
Observation Table
A. Qualitative test
B. Semi-Quantitative Test
Conclusion :
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Clinical
Significance
Most of the strains
of streptococci which are pathogenic for human beings belong to Group A. All
such organisms produce an exotoxin known as Streptolysin ‘O’ (SLO) besides
other toxins and enzymes.When infected with infection, a patient will produce
an antibody, anti-Streptolysin ‘O’ (ASO) that is detectable in serum.Increased
ASO titers may be associated with Rheumatic fever and Glomerulonephritis.An elevated ASO titer of
more than 200 IU/mL indicates past or present acute streptococcal infection.
The
titer of ASO should be observed repeatedly over a time of 4 to 6 weeks to
follow the course of an injection.ASO Antistreptolysin ‘O’ Latex Test Kit
provides a simple screening method of detecting an elevated ASO titer and
obviates the necessity of performing a cumbersome and time hemolytic inhibition
test in all patients.
Reference
KIT USED – ARKRAY HEALTHCARE PVT. LTD.
ASO ANTISTREPTOLYSIN
‘O’ LATEX TEST KIT